Fig. 1

The location sites of 5 SNPs and a loss of nucleotide in p53 genomic DNA. a Schematic diagram of the p53 genomic DNA, the NCBI association number of which is NC_000017.10. The numbered boxes indicate different exons. We designed 4 pairs of primers (P1 to P4) to analyze the DNA sequence. We found 5 SNPs and a loss of 16-bp nucleotides, and assigned these variants the numbers 1–6. b Electropherogram of the 4 sections (P1-P4) of the p53 gene amplified via polymerase chain reaction (PCR) from a GBM tumor specimen. The PCR product sizes are indicated below each of the images. c The detailed sequences and locations of 4 SNPs in P1-PCR production. The red colored sequences of letters indicate exons, and the gray colored sequences of letters indicate introns. The numbers 1 to 4 indicate, respectively, SNP38 (No. 1), the 16-bp duplication polymorphism (rs17878362) (No. 2), SNP11299 (No. 3), and codon 72 (No. 4). The sequences of letters underlined in blue indicate primers. The sequence of letters underlined in green indicates the 16-bp duplication polymorphism. The sequences of letters underlined in purple indicate 4 9-bp repeats. d The detailed sequences and location of 2 SNPs in P3-PCR production. The red colored sequences of letters indicate exons, and the gray colored sequences of letters indicate introns. The numbers 5 and 6 indicate SNP72 (No. 5) and SNP92 (No. 6), respectively