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Table 1 Selective antagonists of 5-HT5A inhibit sphere formation by human breast tumor cells

From: Antagonists of the serotonin receptor 5A target human breast tumor initiating cells

Compound Cell Line IC50 (μM)
Sphere Formation PrestoBlue Reduction
SB-699551 HCC1954 0.3 0.2
MCF-7 0.2 0.2
G1A-(S) HCC1954 2.5 6.7
MCF-7 2.5 3.0
G1A-(R) HCC1954 16.4 14.2
MCF-7 11.0 21.9
G1B-(S) HCC1954 3.0 3.8
MCF-7 2.2 1.3
G1B-(R) HCC1954 11.3 11.8
MCF-7 11.4 14.1
AS2030680 HCC1954 1.8 3.9
MCF-7 2.0 5.6
ASP5736 HCC1954 > 25.0 > 25.0
MCF-7 > 25.0 > 25.0
  1. Tumorsphere derived cells were exposed to each of 7 concentrations of each compound or its vehicle and then incubated for 72 h prior to sphere forming and PrestoBlue reduction assays. The IC50 values (in μM) were calculated by nonlinear regression of dose-response curves using GraphPad Prism 7.0. A minimum of three technical replicates were included for each concentration of the compounds