Skip to main content

Table 1 Selective antagonists of 5-HT5A inhibit sphere formation by human breast tumor cells

From: Antagonists of the serotonin receptor 5A target human breast tumor initiating cells

Compound

Cell Line

IC50 (μM)

Sphere Formation

PrestoBlue Reduction

SB-699551

HCC1954

0.3

0.2

MCF-7

0.2

0.2

G1A-(S)

HCC1954

2.5

6.7

MCF-7

2.5

3.0

G1A-(R)

HCC1954

16.4

14.2

MCF-7

11.0

21.9

G1B-(S)

HCC1954

3.0

3.8

MCF-7

2.2

1.3

G1B-(R)

HCC1954

11.3

11.8

MCF-7

11.4

14.1

AS2030680

HCC1954

1.8

3.9

MCF-7

2.0

5.6

ASP5736

HCC1954

> 25.0

> 25.0

MCF-7

> 25.0

> 25.0

  1. Tumorsphere derived cells were exposed to each of 7 concentrations of each compound or its vehicle and then incubated for 72 h prior to sphere forming and PrestoBlue reduction assays. The IC50 values (in μM) were calculated by nonlinear regression of dose-response curves using GraphPad Prism 7.0. A minimum of three technical replicates were included for each concentration of the compounds