Fig. 6
From: Therapeutic effect of dual CAR-T targeting PDL1 and MUC16 antigens on ovarian cancer cells in mice
Three cytokines production by effector cells in response to various OVCAR-3 cell lines. Four effector cells (PD1-antiMUC16 CAR-T cells, PD1 CAR-T cells, anti-MUC16 CAR-T cells and T cells without CAR molecule) co-cultured with four kinds of OVCAR-3 cells (OVCAR3-luc cells, OVCAR3-MUC16-GFP-luc cells, OVCAR3-PDL1-luc cells, and OVCAR3-MUC16-GFP-PDL1-luc cells) at 1:1 ratio for 48 h to explore the different secretion capacities of IL-2, IFN-γ, and TNF-α between four T cells. These cytokines tested by ELISA kits. Only T: without target cells, O: OVCAR3-luc cell, O-M: OVCAR3-MUC16-GFP-luc cell, O-P: OVCAR3-PDL1-luc cell, O-M-P: OVCAR3-MUC16-GFP-PDL1-luc cell, −:The groups covered by horizontal line are compared in pairs. Data show the mean ± SD, and the results were analyzed with the nonparametric test. Error bars represent the SD.ns: P > 0.05, *: p < 0.05