Fig. 5
From: Therapeutic effect of dual CAR-T targeting PDL1 and MUC16 antigens on ovarian cancer cells in mice
The antitumor activity results in single and dual CAR-T cells against various target cells. There are four kinds of tumor cells expressing PDL1 and(or) MUC16 antigen or not, and four different T cells expressing PDL1 and(or) antiMUC16 or not. The CAR-T cells co-cultured for 4 h with target cells (1 × 104) at E/T of 1:1,4:1,8:1, and 16:1 in a total volume of 100ul, after that stained with 70 μL Steady-Glo for 20 min and detected by flow cytometry. Data show the mean ± SD, and the results were analyzed with the nonparametric test. Error bars represent the SD. ns: P > 0.05, *: p < 0.05