Fig. 1
From: Therapeutic effect of dual CAR-T targeting PDL1 and MUC16 antigens on ovarian cancer cells in mice
Construction of dual CAR molecule and PD1-antiMUC16 plasmid. a Schematic diagram of dual-target CAR molecule transgene. PD1-antiMUC16 CAR structure shows the five parts, extracellular PD1-antiMUC16 scFv, a CD8a hinge, a transmembrane region, 4-1BB co-stimulation domain, and CD3ζdomain, among the structure anti-MUC16 that consist of 4H11-VH and 4H11-VL. b Schematic diagram of dual-target CAR expression vector. PD1-antiMUC16 plasmid structure shows base sequence sites and the sites of enzyme digestion