Fig. 3

Effect of NSC 23766 and ML141 on the invasion ability of pancreatic cancer cells. Pancreatic cancer cell lines (BxPC-3, Panc-1, HPAFII) were treated with either NSC 23766 (0, 50, 100, 200 μM) or ML141 (0, 10, 20, 40 μM), and incubated for 24 h. a-c, g-i Migrated cells were visualised via phase-contrast microscopy. d-f, j-l % wound closure was measured, and the ratio to wound width at the start of the culture was used as an index of cell migration to conduct a comparative evaluation against the control group (n = 3). Data are presented as mean ± SD. *P < 0.05, **P < 0.01 vs control