Fig. 9

PT induces apoptosis by activating p38 MAPK. a HT-29 b SW620 cells were pre-treated with p38 MAPK inhibitor SB202190 (10 μM) for 1 h and then exposed to PT for 24 h at 37 °C. Cytosolic fraction were prepared and immunoblotted for the indicated antibodies. c SW620 cells were transfected with control and p38 siRNA duplex for 48 h then exposed to PT for 24 h at 37 °C. Total cell lysate were prepared and immunoblotted with indicated antibodies. Density of the protein bands of three independent experiments were quantified and expressed as relative protein expression to actin. The bar graphs are presented as mean ± SD of three independent experiments. **p < 0.01, ***p < 0.001 vs control. d SW620 cells were transfected with control and p38 siRNA duplex for 48 h then exposed to PT for 24 h at 37 °C. These cells were incubated with Annexin V/PI and %total cell death was detected by flow cytometry. e Results shown are representative of three independent experiments. ***p < 0.001 Control siRNA+PT vs Control siRNA; *** p < 0.001 p38siRNA + PT vs Control siRNA+PT. Full Length blots were presented in Supplementary Figure S4