Fig. 3

PT alters redox balance. a HT-29 b SW480 c SW620 cells were exposed to PT for 24 h. Cells were incubated with 2′,7′–dichlorofluorescein diacetate (DCFDA) for 15 min and the fluorescence of the oxidized 2′,7′–dichlorofluorescein was detected by flow cytometry. c HT-29 d SW480 e SW620 were treated with PT for 24 h. The absorbance was detected at 405 nm using plate reader. The bar graphs are presented as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs control