Fig. 3

Knockdown of Dazl inhibited glioblastoma cell migration and invasion in vitro. a Cell migration assays were performed after Dazl deletion in A172, U251, and LN229 cells. A172, U251, and LN229 cells with Dazl knockdown exhibited decreased ability to migrate through the Boyden chamber compared with the WT GMB cell lines. Five pictures were collected for each group, and the representative images were shown here. Images were taken with an inverted microscope (bars = 50 μm, magnification × 200). b The statistical analysis of the ability of the glioblastoma cells’ migration (^**P < 0.01, ^***P < 0.001). c The invasion of A172, U251, and LN229 cells with Dazl knockdown was measured by transwell assay. Cells migrated through Matrigel-coated transwell inserts and relative invasion proportion of cells were shown. Five pictures were collected for each group, and the representative images were shown here. Images were taken with an inverted microscope (bars = 50 μm, magnification × 200). d The statistical analysis of the ability of the glioblastoma cells’ invasion, the numbers of invasion cells with Dazl knockdown were significantly less than those with untreated cells. All experiments were carried out in triplicate. Data are shown as the mean ± SE (^*P < 0.05, ^***P < 0.001)