Fig. 2

Dazl knockdown inhibited the proliferation and germline traits of glioblastoma cells in vitro. a Western blot analysis detected whether Cas9 protein was transfected into GBM cells successfully and whether Dazl protein was deleted. b A CCK-8 cell proliferation assay was performed after Dazl deletion in A172, U251, and LN229 cells. c An alkaline phosphatase stain assay was performed between the WT GBM cell lines and the Dazl deletion cells. Images were taken from the inverted microscope (magnification × 200). All experiments were carried out in triplicate. Data are shown as the mean ± SE (^*P < 0.05, ^**P < 0.01)