Skip to main content
Fig. 1 | BMC Cancer

Fig. 1

From: LINC00992 contributes to the oncogenic phenotypes in prostate cancer via targeting miR-3935 and augmenting GOLM1 expression

Fig. 1

LINC00992 was overexpressed in prostate cancer and regulates cell proliferation, apoptosis and migration. a GEPIA database demonstrated the overexpression of LINC00992 in tumor tissues in contrast to adjacent normal ones. b LINC00992 expression was detected by qRT-PCR in four prostate cancer cell lines and control RWPE-1 cells. c LINC00992 expression was monitored by qRT-PCR in DU145 and PC3 cells after transfection with shRNAs targeting LINC00992. sh-NC was used as the negative control. d The viability of DU145 and PC3 cells was estimated through CCK-8 assay following LINC00992 depletion. e The proliferation of DU145 and PC3 cells was investigated after LINC00992 depletion via EdU assay. Scale bar = 200 μm. f The apoptosis of DU145 and PC3 cells transfected with sh-LINC00992#1/2 or sh-NC was estimated via TUNEL assay. Scale bar = 200 μm. g Western blot analysis was applied to examine the expression of apoptosis-related proteins. h-i The migration of DU145 and PC3 cells was analyzed via Transwell migration assay (scale bar = 100 μm) and wound healing assay (scale bar = 100 μm) after inhibiting LINC00992 expression. The full-length images for blots in Fig. 1g were presented in Supplementary figure 4. *P < 0.05, **p < 0.01

Back to article page