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Fig. 2 | BMC Cancer

Fig. 2

From: Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy

Fig. 2

The interaction of ADI and FTL in vivo. a: Yeast were co-transformed with pBD-ADI and pAD-T-FTL plasmid, and grew on an SD agar plate with high-stringency nutrient selection (SD/−Leu/−Trp/−His/−Ade). pBD-LamC/pAD-T-antigen plasmids were used as negative control. pBD-p53/pAD-T-antigen plasmids were used as positive control. b: Co-IP of ADI or FTL was applied by antibodies specific for ADI or FTL. Images represent the immuneprecipitates separated by SDS-PAGE and incubated with the indicated antibodies. The blots of each line were from the same gel. Full-length blots are presented in Supplementary Fig. S2. c: Immunofluorescence staining of HepG2 and PC3 cells with antibody against ADI (red) and antibody against FTL (green). Cells were transfected with pcDNA4-ADI plasmid. The fluorescence was detected on an inverted fluorescence microscope

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