Fig. 4

Ruxolitinib therapy causes loss of CSF3R mutated clone, cell size normalization, but only marginally compensates the migration defect of aCML neutrophils. a Variant allele frequency (VAF) of candidate genes of the aCML patient over the course of ruxolitinib therapy. b Changes in the migratory patterns, percentage of moving cells (left) and speed excluding non-moving cells (speed excl. Nmc., right), of the aCML neutrophils over the course of treatment. From top to bottom, cells were stimulated with PBS, fMLP [10 nM], CXCL1 [100 ng/ml] and CXCL8 [100 ng/ml]. Black triangles and black solid lines indicate aCML neutrophils (every timepoint n = 1), while grey dots and grey dashed lines indicate the median and the grey dotted lines indicate the interquartile range of the age- and gender-matched controls (n = 6). Numbers label the specific values of aCML neutrophils reached for percentage of moving cells (left) and speed excluding non-moving cells (right) respectively. c Changes in cell size of aCML neutrophils under the four different stimulation conditions over the course of therapy. Black triangles and black solid lines indicate aCML neutrophils (every timepoint n = 1), while grey dots and grey dashed lines indicate the median and the grey dotted lines indicate the interquartile range of the age- and gender-matched controls (n = 6). On average, 41 and 56 cells per condition were analyzed in age- and gender-matched controls and the aCML patient, respectively. Numbers label the specific cell sizes as the mean cell size of all cells