Fig. 5

IMMUNEPOTENT CRP induces autophagosome formation in HeLa and MCF-7 cells. a. HeLa, and b. MCF-7 cells were left alone or pretreated with NAC before ICRP treatment (24 h), and observed by optical microscopy (10X). c, d. Autophagosome formation was determined by flow cytometry through CYTO-ID staining in HeLa (c) and MCF-7 cells (d) that were left alone or pretreated with NAC before ICRP treatment (24 h). e. The results were analyzed using Flowjo software and graphed. Graphs shown are means (± SD) of triplicates of three independent experiments