Fig. 3

IMMUNEPOTENT CRP induces eIF2α phosphorylation and CRT exposure in HeLa and MCF-7 cells. a, b eIF2α phosphorylation (P-eIF2α) in HeLa (a) and MCF-7 (b) cells measured by flow cytometry. Negative controls, with IgG isotype antibodies, are shown in dotted, and in gray Control (without treatment) and ICRP treatment. c, d. Confocal microscopy representation of P-eIF2α in HeLa (c) and MCF-7 (d) cells. Representative diagrams of surface CRT detection in HeLa (e) and MCF-7 (f) cells using FACS. Negative controls, with IgG isotype antibodies, are shown in dotted, and in gray Control (without treatment) and ICRP treatment. CRT exposure in HeLa (g) and MCF-7 (h) cells was observed after treatment with ICRP by CRT-PE staining and visualized by confocal microscopy. Graphs represent the means (± SD) of triplicates of at least three independent experiments