Fig. 1

IMMUNEPOTENT CRP induces RCD through mitochondrial and cell cycle alterations in HeLa and MCF-7 cells. a. Cell death was measured by flow cytometry through Annexin-V and PI staining and graphed, in HeLa (left) and MCF-7 cells (center), and PBMC (right) treated with different concentrations of ICRP for 24 h. b. Loss of ΔΨm induced by ICRP was measured by flow cytometry using TMRE staining in HeLa (1.25 U/mL, 24 h) (left), MCF-7 cells (1.5 U/mL, 24 h) (center), and PBMC (1.5 U/mL, 24 h) (right). c. ROS levels were measured by flow cytometry through DCFDA staining in HeLa (1.25 U/mL, 24 h) (left), MCF-7 cells (1.5 U/mL, 24 h) (center), and PBMC (1.5 U/mL, 24 h) (right). d. Schematic representation of changes in cellular DNA content measured by flow cytometry through PI staining in HeLa (1.25 U/mL, 24 h) (left) and MCF-7 cells (1.5 U/mL, 24 h) (right). e. The results obtained as in (D) were analyzed using Flowjo software and graphed. The graphs represent the means (± SD) of triplicates of at least three independent experiments