Skip to main content
Fig. 2 | BMC Cancer

Fig. 2

From: The natural alkaloid Jerantinine B has activity in acute myeloid leukemia cells through a mechanism involving c-Jun

Fig. 2

Induction of apoptosis in IC50JB exposed AML cells.a. Flow cytometric analysis of Annexin V/propidium iodide staining following IC50-JB treatment for 24 and 72 h. Representative flow cytometry plots and summary histograms are shown. A+/PI− indicates cells undergoing early stage apoptosis, while A+/PI+ defines late stage apoptotic populations. b. Summary bar chart of flow cytometric analysis of cleaved PARP and active caspase 3 apoptotic markers following 24 h IC50 JB exposure. c. BH3 profiling following 4 h IC50-JB treatment in MV4–11 cells. Cytochrome C release demonstrates PUMA- and BAD-BH3 peptides prime the cells to apoptosis. PUMA2A is a mutated peptide which acts as a negative control . Columns, mean of at least three independent experiments; bars, SD. * P < 0.05, ** P < 0.01, *** P < 0.001

Back to article page
\