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Fig. 3 | BMC Cancer

Fig. 3

From: Starvation stress attenuates the miRNA-target interaction in suppressing breast cancer cell proliferation

Fig. 3

Luciferase reporter assays demonstrated the attenuated interaction between miR-221/222 and p27 by starvation stimulation. a: Schematic representation of the luciferase reporter structure carrying human p27 3’UTR. b: Luciferase reporter assay demonstrating the direct interaction between p27 3’UTR and miR-221/222 under regular cell culture condition. c: Luciferase reporter assay demonstrating the attenuated interaction between p27 3’UTR and miR-221/222 under starvation conditions. d: Western blot showing downregulated E2F1 and cyclin D1, two downstream genes of p27, by knockdown of miR-221/222 under normal cell culture condition. e: Western blot showing the expression of E2F1 and Cyclin D1 was not affected by miR-221/222 under starvation condition. Original gels for western blots were shown in Supplemental Fig. S6. Data are presented as mean ± SEM (n = 4), ***p < 0.0001

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