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Fig. 2 | BMC Cancer

Fig. 2

From: Starvation stress attenuates the miRNA-target interaction in suppressing breast cancer cell proliferation

Fig. 2

Starvation stimulation attenuated the miR-221/222-p27 target interaction in regulating cell proliferation in MDA-MB-231. a: Two binding sites of miR-221/222 were identified in the highly conserved 3’UTR region of p27 mRNAs. b, c: Sequence alignment between conserved 3’UTR of p27 mRNA and miR-221/222. The two binding sites were highlighted and boxed. d: MTT assays showing decrease of cell proliferation in MDA-MB-231 cells after the knockdown of miR-221 and/or miR-222, compared to negative control (anti-NC). e: Western blot analysis showing increased p27 protein level after addition of anti-miR-221 and/or anti-miR-222 into MDA-MB-231 cells under regular cell culture condition, but not under starvation condition. f: Western blot showing suppression of p27 at the protein level by overexpression of miR-221 and/or miR-222 in MDA-MB-231 cells under regular cell culture condition, but not under starvation condition. β-actin served as loading control. Original gels for western blots were shown in Supplemental Fig. S6. Data are derived from three independent analyses, and presented as mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.0001

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