Fig. 1

Starvation stimulation suppressed cell proliferation in human breast cancer. a: MTT assays showing decreased cell proliferation of MDA-MB-231 cells by starvation stimulation, compared to regular cell culture condition. b: Western blot analysis demonstrating the increased p27 expression by starvation stimulation in MDA-MB-231 cells. c: Western blot analysis demonstrating the decreased Cyclin D1 expression under starvation in MDA-MB-231 cells. β-actin served as loading control. Original gels for western blots were shown in Supplemental Fig. S6. Data are derived from three independent analyses, and presented as mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.0001