Fig. 4

Effect of the checkpoint HLA-G/ILT4 on the expression of VEGF genes. RNA prepared from RCC7-wild type (wt), RCC7-HLA-G1, RCC7-ILT4, M8- wild type (wt) and M8-HLA-G1 cell lines were subjected to semi-quantitative RT-PCR using specific primers. The symbol (***) shows that differential expression is statistically significant (p < 0,01). a HLA-G1 expression in the three cell lines; b ILT4 expression in the three cell lines; c VEGF-A expression in the three cell lines; d VEGF-C expression in the three cell lines ;  e HLA-G expression in M8 (wt) and M8-HLA-G1 and f VEGF-C expression in M8-(wt) and M8-HLA-G1.