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Fig. 4 | BMC Cancer

Fig. 4

From: Etoposide-mediated interleukin-8 secretion from bone marrow stromal cells induces hematopoietic stem cell mobilization

Fig. 4

Mouse model of peripheral blood hematopoietic stem cell mobilization. a, b, c The mouse model of hematopoietic stem cell (HSC) mobilization was designed based on a protocol used in human patients (G-CSF only, n = 8; cyclophosphamide + G-CSF, n = 8; etoposide + G-CSF, n = 8). d On day 7 (D7) of the protocol, HPCs were isolated from the peripheral blood and CFUs (CFU-granulocytes, erythrocytes, monocytes, and megakaryocytes; CFU-granulocytes, macrophages; and burst-forming unit-erythroid) were counted. The presented pictures were obtained in the control group (G-CSF only). e The cyclophosphamide-treated (total 200 mg/kg) and etoposide-treated (total 80 mg/kg) groups showed a higher number of CFUs than the G-CSF only group (p = 0.021 and 0.003 after Bonferroni correction, respectively). No significant difference was observed in the total number of CFUs between the cyclophosphamide-treated (200 mg/kg) and etoposide-treated (80 mg/kg) groups (G-CSF only, n = 5; cyclophosphamide + G-CSF, n = 5; etoposide + G-CSF, n = 5). Note: ** p < 0.01 after Bonferroni correction; * p < 0.05 after Bonferroni correction. Note: Values are reported as the mean ± SEM. Abbreviations: S.C., subcutaneous injection; I.P., intraperitoneal injection; NS, normal saline; G-CSF, granulocyte colony-stimulating factor; CY, cyclophosphamide; ETO, etoposide; CFU, colony-forming unit; GEMM, granulocytes, erythrocytes, monocytes, and megakaryocytes; GM, granulocytes, macrophages; BFU-E, burst forming unit-erythroid; n.s., not significant

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