Fig. 3

Ex vivo validation screening using different assay techniques. a Heatmap display of vertical unsupervised clustering of the dose response data of the drugs from independent ex vivo screens using a 2D enzymatic cell viability assay, an image-based 2D cell viability assay and an image-based 3D cell viability assay. Each drug was tested in five concentrations. GR values < 0 shown in blue. b Comparison of the assay controls used to calculate growth rates of the patient derived cells in the 2D and 3D culture conditions and measured with the different assay techniques. c Venn diagram showing the overlap of the top 30 most cytotoxic drugs from the different assay techniques. d Curve fitted dose response curves of the most potent cytotoxic drugs across all three different replicate screens. e Topoisomerase inhibitors had systematically a more potent cytotoxic effect in the 3D cell culture model assay