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Fig. 3 | BMC Cancer

Fig. 3

From: MiR-34c downregulation leads to SOX4 overexpression and cisplatin resistance in nasopharyngeal carcinoma

Fig. 3

MiR-34c regulates the SOX2-EMT axis in NPC cell lines. a, b and c Relative expression (qRT-PCR) of SOX2: a in NP69, NP460 and, C666–1 cell lines, normalized to C666–1 cells; b in NP69-anti-miR-34c stable cells and their control; c in NP460-anti-miR-34c stable cells and their control. d and e Western blots (WBs) were performed using anti-Snail (αSnail), anti-Claudin-1 (αClaudin-1), anti-ZO-1 (αZO-1), anti-ZEB1 (αZEB1), anti-CDH1 (αCDH1), anti-CDH2 (αCDH2), with anti-β-actin (αβ-actin) as loading control. d WBs using NP69-anti-miR-34c stable cells and their control. Full-length blots are presented in Additional file 6: Figure S6. (E) WBs using NP460-anti-miR-34c stable cells and their control. ZO-1 was undetectable in NP460 cell lines. Full-length blots are presented in Additional file 7: Figure S7. f Relative SOX2 expression assessed by qRT-PCR in C666–1 cells treated with SB431542 (10 or 20 μM). The data are expressed as the mean ± SEM of at least three independent experiments. * P < 0.05; *** P < 0.001

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