Skip to main content

Table 1 Disease-associated clone frequency ratios assessed in linearity study

From: Analytical evaluation of the clonoSEQ Assay for establishing measurable (minimal) residual disease in acute lymphoblastic leukemia, chronic lymphocytic leukemia, and multiple myeloma

 

Total DNA Input

40 μg

20 μg

2 μg

200 ng

Replicates

 

# of cancer cells

6,125,574

3,062,787

306,279

30,628

8

Freq 1

2

 

0.000065%

0.00065%

0.0065%

8

Freq 2

2.5

 

0.000082%

0.00082%

0.0082%

8

Freq 3

3

 

0.0001%

0.001%

0.01%

4

Freq 4

4.6

0.000075%

   

3

Freq 5

6.1

0.0001%

   

4

Freq 6

9.2

 

0.0003%

0.003%

0.03%

2

Freq 7

30.6

 

0.001%

0.01%

0.1%

4

Freq 8

61.3

0.001%

   

2

Freq 9

91.9

 

0.003%

0.03%

0.30%

2

Freq 10

306.3

 

0.01%

0.10%

1%

3

Freq 11

612.6

0.01%

   

2

Freq 12

918.8

 

0.03%

0.30%

3%

2

Freq 13

3062.8

 

0.1%

1%

10%

4

Freq 14

6125.6

0.1%

   

2

Freq 15

9188.4

 

0.30%

3%

30%a

2

Freq 16

30,627.9

 

1%

10%

100%a

4

Freq 17

61,255.7

1%

   

2

Freq 18

91,883.6

 

3%

30%a

 

2

Freq 19

306,278.7

 

10%b

100%a

 

4

Freq 20

612,557.4

10%b

   
  1. Freq frequency
  2. 1 human diploid cell = 6.53 pg
  3. aSingle cell line in test, not mixed with other cell lines
  4. b3 Cell lines for each cancer type were combined; then CLL, MM, and ALL were tested separately