Fig. 5

CCL18-NIR1 axis promoted the locomotion and EMT of OSCC cells. HSC6 cells and CAL27 cells were treated with rCCL18, siNC+rCCL18 and siNIR1 + rCCL18 respectively. The untreated cells and siNC+rCCL18 cells were used as control. a and b The effects of CCL18-NIR1 axis on the migration and invasion of OSCC cells. Oral cancer cells at the bottom of the transwell membrane were stained with 0.4% crystal violet. Five fields per hole were randomly selected under the microscope at 50×. c The protein expression of E-cadherin, N-cadherin and ZEB2 in HSC6 and CAL27 cells which subjected to the different treatment stated above were tested by western blotting, β-actin was used as the internal control. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. control). The full-length blots are presented in Supplementary Fig. S4