Fig. 1

NIR1, which is a potential receptor of CCL18, was shown to be highly expressed in OSCC. a Representative images of NIR1 staining in OSCC tissues and adjacent normal tissues (magnification 100×). b Western blotting results showed that the protein level of NIR1 is up-regulated in oral cancer tissues (**P < 0.01, ***P < 0.001 vs. adjacent normal tissues). c and d qRT-PCR assay and western blotting showed that NIR1 was overexpressed in OSCC cell lines (i.e., HSC6, CAL27, SCC9) compared with HOK cells (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. HOK). The data represent mean ± SEM of three independent experiments. e Immunofluorescence staining of NIR1 (red) and nuclei (blue) in oral cancer cells (i.e., HSC-6, CAL27 and SCC9) and HOK cells (magnification 200×). f IHC images showed that NIR1 and CCL18 more expressed in metastatic cases of OSCC than in non-metastatic cases (magnification 50×). The full-length blots are presented in Supplementary Fig. S1