Fig. 5

BMS-191011 induced activation of caspase-3 in MDA-MB-231. Using caspase-3/7 green dye, fluorescence was detected after incubation of 10 μM BMS-191011 for three days (b). Light image is shown in (a). Scale bar: 20 μm. Control cells are shown in the Supplemental Fig. 14. Using immunoblots, caspase-3 was detected in three TNBC cell lines (MDA-MB-231, HCC1143, SUM159) (c). Cleaved caspase-3 was detected after BMS-191011 treatment (d, M-marker, red arrow indicates pro-caspase 3, black arrow indicates cleaved active caspase-3). Figure 5c and d were prepared by cropping the full WB in Supplemental Figs. 17 and 18, respectively, by removing the white spaces above and below the signals of interest