Fig. 2

Protein expression of BK channel α-subunit (forming the functional channel) in TNBC cells and patient tissues. a Western blots with antibody, M: marker, MDA231: MDA-MB-231 cells (cell positive control), Normal: normal primary breast tissue, TNBC: TNBC patient tissue, MB: mouse brain (tissue positive control). β-actin was used as a loading control. b Western blots with antibody and antigen, confirming signals detected by the antibody in (a). Figure 2a and b were prepared by cropping the full WB shown in Supplemental Fig. 15 by removing the white spaces above and below the signals of interest. c Quantitative expression levels of BK channel proteins normalized to β-actin (n = 4–6). Unpaired t test was performed. Two-tailed p = 0.0002, t = 0.8172. d Western blots using a BK channel protein antibody in MDA231, SUM159, MCF10A, and HCC1143, β-actin as loading controls. Figure 2d was prepared by cropping the full WB in Supplemental Fig. 16 by removing the white spaces above and below the signals of interest