Prognostic significance of branched-chain amino acid transferase 1 and CD133 in triple-negative breast cancer

BMC Cancer

Table 1 Immunohistochemical staining standards for the pathological biomarkers

Antibody	Clone	Dilution	Source	Positive style	Positive control	Cutoff values (%)	Antigen retrieval	Incubation
Erα	Rabbit monoclonal	Prediluted	Epitomics	Nuclear staining	Breast cancer	≥1	100 °C, 30 min	37 °C, 32 min
PR	Rabbit monoclonal	Prediluted	Epitomics	Nuclear staining	Breast cancer	≥1	100 °C, 30 min	37 °C, 32 min
HER-2	Rabbit monoclonal	Prediluted	Ventana	Membrane staining	Breast cancer	≥3+ or FISH+	100 °C, 30 min	37 °C, 32 min
CK5/6	Mouse monoclonal	Prediluted	Dako	Membrane staining and/or cytoplasmic staining	Mesothelioma	≥5	100 °C, 31 min	37 °C, 32 min
EGFR	Rabbit monoclonal	Prediluted	Ventana	Membrane staining and/or cytoplasmic staining	Skin	≥25	100 °C, 32 min	37 °C, 32 min
P53	Mouse monoclonal	Prediluted	MXB	Nuclear staining	Colon adenocarcinoma	≥5	100 °C, 33 min	37 °C, 32 min
Ki-67^a	Mouse monoclonal	Prediluted	ZSGB-BIO	Nuclear staining	Breast cancer	≥14	100 °C, 34 min	37 °C, 32 min
BCAT1	Rabbit polyclonal	1:100	Absin	Cytoplasmic staining	hepatocellular cancer	≥90	100 °C, 35 min	37 °C, 32 min
CD133	Rabbit Monoclonal	1:400	CST	Membrane staining and/or cytoplasmic staining	Lymphnodes	≥95	100 °C, 36 min	37 °C, 32 min

^aKi-67 index threshold of 14% was chosen according to the St. Gallen Consensus 2013

ISSN: 1471-2407