Fig. 7
OTUD3 induces apoptosis in BC cells and inhibits colony formation. a Cell apoptosis detection: negative control and OTUD3 and OUUD3C76A vectors were transfected into MCF7 and DU4475 cell lines. The cells were then subjected to an apoptosis detection. b OTUD3 knockdown by shRNA and OTUD3 and OTUD3C76A restoration without shRNA interference for cell apoptosis detection. c-d Cell clone formation detection: OTUD3 negatively regulates cell clone formation in MCF7 cells and DU4475 cells. The data are presented as the mean ± standard deviation of three independent experiments. *p < 0.05, **p < 0.01