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Fig. 3 | BMC Cancer

Fig. 3

From: Tumor suppressor OTUD3 induces growth inhibition and apoptosis by directly deubiquitinating and stabilizing p53 in invasive breast carcinoma cells

Fig. 3

OTUD3 stabilizes p53. a MCF7 cells and DU4475 cells transfected with an OTUD3-expressing vector or control (Flag) vector were analyzed to detect the indicated proteins by immunoblotting (IB). b MCF7 cells and DU4475 cells transfected with sh-ctrl or sh-OTUD3 lentivirus were treated with or without the proteasome inhibitor MG132 (20 μM, 8 h) and then analyzed to detect the indicated proteins. c Half-life analysis of p53 in MCF7 cells and DU4475 cells. d Protection of p53 from Mdm2-mediated degradation by OTUD3 in BC cells

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