Fig. 5

Knockdown of RABL6 inhibited epithelial-mesenchymal transition in ESCC cells (a-c). a-b Relative expressions of E-cadherin, a-catenin, b-catenin, Vimentin, and Slug in YES-2 and TE-2 cells were compared by qRT-PCR between siRNA RABL6-silenced cells (si#-1, si-#2) and their control cells (si-N). c Western blots comparing siRNA RABL6-silenced cells (si#-1, si-#2) with their control cells (si-N) are showed with the expression of vimentin, slug, β-catenin and E-cadherin. A-tubulin was taken as control; the original full-length gels are presented in Supplementary Figure S2. d Representative bright-field images of ESCC cells and ESCC cells stained with fluorescent phalloidin to show actin structures, with or without silencing of RABL6. DAPI (blue) was applied to stain nuclear DNA. (original magnification of bright-field: 400x; original magnification of fluorescent phalloidin: 1000x). Data are expressed in the form of the mean ± SEM. * indicates P < 0.05, ** indicates P < 0.01 versus the control