Fig. 1

RABL6 is highly-expressed by ESCC and is associated with worse prognosis. aRABL6 expression was tested and compared between normal esophageal epithelial cell line NE-1 and Het-1 and all tested ESCC cell lines via qRT-PCR. b Expression of RABL6 in different ESCC cell lines and in normal esophageal epithelial cell line Het-1A was compared by Western blot analysis; the original full-length gels are presented in Supplementary Figure S1. c-d The scores of RABL6 expression in 171 ESCC cancerous samples were compared with matched non-cancerous samples by immunohistochemistry staining. c Representative images of RABL6 IHC staining in 2 pairs of ESCC cases (original magnification: 100x). d Results are expressed in the form of mean+/−SEM. e- f Kaplan-Meier analysis showed the overall survival rate and disease-free survival rate of ESCC patients stratified by RABL6 expression. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.01 for statistical results