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Fig. 2 | BMC Cancer

Fig. 2

From: Sophoridine exerts tumor-suppressive activities via promoting ESRRG-mediated β-catenin degradation in gastric cancer

Fig. 2

SOP induces apoptosis and G2/M phase arrest in gastric cancer cells. a AGS cells were treated with indicated concentrations of SOP for 24 h, Annexin V-FITC/PI stain and flow cytometry analysis were performed to assess apoptosis. b Statistical analysis of the Annexin V+PI+ cell ratio in AGS cells. c Statistical analysis of the Annexin V+PI+ cell ratio in SGC7901 cells. d Western blot analysis of the expression of apoptosis related proteins in AGS cells treated with indicated concentrations of SOP for 24 h. Full-length blots/gels are presented in Supplementary Figure S4. e AGS cells were treated with indicated concentrations of SOP for 24 h, PI stain and flow cytometry analysis were performed to assess cell cycle distribution. f Statistical analysis of cell cycle phase ratio in AGS cells. g Statistical analysis of cell cycle phase ratio in SGC7901 cells treated with indicated concentrations of SOP for 24 h. h AGS cells were treated with or without 3 μM SOP for indicated hours, and then γH2AX and RAD51 expression were determined by western blot. Full-length blots/gels are presented in Supplementary Figure S4. The results are representatives of at least 3 independent experiments. Data were presented as mean ± SD. Abbreviation: SOP, Sophoridine

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