Fig. 6

Nutlin-3 + PX-478 and DCA + NHI-2. MCF-7 cells were seeded into a 96 well plate at a density of 0.5 × 10⁴/well (MCF-7), incubated 24 h to a confluency of 50%, then incubated with increasing concentrations of Nutlin-3, PX-478 and their combination (a, b) as well as DCA, NHI-2 and their combination (c, d) and) for 48 h. Then, viability was assessed using MTT assay (a, c) and protein mass was assessed using SRB assay (b, d). CI-Values were calculated by CompuSyn and illustrated with red dots in the diagram on the right. Each dot corresponds to the respective combination shown in the graph to the left. The effects of EC₅₀of DCA, NHI-2 and DCA + NHI-2 on the cell confluency is illustrated on the bottom (e)