Fig. 4

minimalistic drug interaction screening. HT-29 and MCF-7 cells were seeded into a 96 well plate at a density of 1.5 (HT-29) or 0.5 × 10⁴/well (MCF-7) and incubated 24 h to a confluence of 50%. Then, cells were incubated with 14 single compounds and the respective 91 combinations at a concentration about EC₂₅ for 48 h. Viability was assessed using the MTT-Assay and the CSP (conjectured synergistical potency) values were calculated. CSP of a combination was quantified by adding up the effect of the single compounds and subtracting the result from the combination’s effect. All CSP values above ten are highlighted in green. Values between ten and 15 are marked by one plus (+), between 15 and 25 by two plus (++), greater than 25 by three plus (+++) and referred to as “possible”, “likely” and “very likely” synergism respectively. The number of total “+” is given in the first column below the name of the compounds and summarizes the number and strength of projected synergistic interactions