Fig. 2

Mig-6 overexpression increased the viability and migration of PC9 cells. a and b Mig-6 expression was examined by western blot and immunofluorescence staining of endogenous Mig-6 in PC9 cells and Mig-6-overexpressing PC9 cells. c The cell viability of PC9 and Mig-6-overexpressing PC9 cells was measured using the Cell Counting Kit-8 (CCK-8) assay. The optical density values were detected on a microplate reader at 450 nm. d Cell migration ability was tested using wound-healing and transwell migration assays. Boyden chambers were stained with crystal violet for the transwell migration and invasion assays. The number of cells was counted under a microscope (*P < 0.05, **P < 0.01). e The viability of cells treated with different EGFR-TKI doses was measured using the CCK-8 assay. f PC9 cells and Mig-6-overexpressing PC9 cells were incubated with medium alone (control) or various concentrations of gefitinib for 24 h, and cleaved PARP/PARP expression was assessed by western blot analysis. g Proteins related to epidermal growth factor receptor (EGFR) signaling were checked with Western blot in PC9 and Mig-6 overexpressed PC9 cells. The expressions Mig6, p-EGFR (Tyr1068), p-EGFR (Tyr1045), EGFR, C-MET, p-AKT, AKT, p-ERK, ERK, E-cadherin, Zo-1 and Vimentin were evaluated. β-actin was used as a loading control. Uncropped blots were shown in Additional file 1: Fig. S2