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Fig. 3 | BMC Cancer

Fig. 3

From: Altered staining patterns and expression level of Engrailed-2 in benign prostatic hyperplasia and prostate Cancer predict prostatic disease progression

Fig. 3

Representative EN2 immunohistochemical images of PC and BPH. a. Representative PC and BPH slices. The upper 3 were BPH slices, the lower 3 were PC slices. Zoom in × 40. b. Two representative BPH slices with strong staining of EN2 and one negative BPH slice with isotype antibody. The staining patterns of EN2 in the left panel was mainly cytomembrane staining, with clear boundaries and sharp contours among cells. There was shallow staining inside the cells, but nuclear membrane of glandular epithelial cells in the basal part of the prostate gland was obviously stained. “I” was neovascularization endothelial membrane stained with EN2 antibody. “II” was glandular epithelial membranes, nucleus and nuclear membrane (indicated by the red arrow) stained with EN2 antibody. In the middle panel, strong staining in glands was shown. “III” was glandular epithelial cytoplasm stained with EN2 antibody. “IV” was nuclear staining in interstitial tissue. The positive staining cells with round nucleus confirmed by the HE staining were infiltrating lymphocytes. In the right panel, negative BPH slice stained with an isotype antibody. c. Two representative PC slices stained with EN2 antibody and one negative PC slice stained with isotype antibody. The EN2 staining sites were mainly focused on the glandular epithelial membrane, glandular cells were well-defined honeycomb-like. “V” and “VI” were cytomembrane staining of EN2 on glandular epithelium cells. “VII” was nuclear membrane staining of EN2 on glandular epithelium cells (indicated by the red arrow). “VIII” was EN2 staining on tumor neovascularization endothelial cells

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