Fig. 5

LBP1A knockdown in HeLa cells results in limited growth reduction, and minimal mitotic delay with no apparent mitotic defects. a-b Asynchronous HeLa cells were transfected with 20 nM of control siRNA or siRNAs targeting either LSF or LBP1A. a Total cell number was measured simultaneously with viability at 120 h post transfection. Data points are technical replicates and means from a single experiment; results are representative of three independent experiments. b Cells were analyzed at the indicated times for viability by the Promega MTT assay. The data are represented as the percentage of viability, compared to that of the control siRNA. Data points and means are derived from 2 independent experiments. **p = 0.0064 (LSF vs. control siRNA, unpaired T test). c HeLa cells transfected with 20 nM LBP1A or control siRNAs were harvested at the indicated times during the cell synchronization protocol using a double thymidine block (see Fig. 3a). Representative immunoblots of LBP1A and β-actin are shown. The images were cropped to indicate the proteins of interest; full images are in Additional file 5. d H2B-YFP-labeled HeLa cells were synchronized with a single thymidine block following transfection with a siRNA targeting either LBP1A or a non-expressed gene as a control. Cells were imaged using time lapse microscopy. Mitotic time was determined by measuring time from nuclear envelope breakdown (NEB) to anaphase. Mitotic times (mean time in minutes +/− standard error, n) for 20 nM control siRNA and 10 nM LBP1A siRNA were: 57.9 +/− 2.8, 101; and 86.6 +/− 10.6, 100; respectively. ** p = 0.009