Fig. 1

in vitro conditioned media from gastrointestinal cell lines influenced DC maturation. a-c Data from DC surface flow cytometry (a) and ELISA of DC supernatants (b-c) was produced from one healthy PBMC donor pre-treated with supernatants of three independent biological replicates of conditioned media harvested from OAC cell lines (green circles) and CRC cell lines (red squares). a HLA-DR was inhibited by conditioned media from the OAC cell line, OE33 Radioresistant, relative to LPS-induced levels (+, grey diamonds), whereas there was inhibition of five DC markers (CD54, CD80, HLA-DR, CD86 and CD83) by conditioned media from one or both of the CRC cell lines. b-c LPS-induced levels of TNF-α was inhibited by OAC and CRC conditioned media (b), though no significant differences were observed for IL-12 p70 (c). DC maturation levels are shown relative to LPS-induced levels in background media (+, grey diamonds) and statistical comparison of in vitro conditioned media+LPS (n = 3) is performed relative to LPS-induction alone (+, n = 3–6). Statistically significant (ANOVA with Dunnett’s Multiple Comparison Test) inhibition of DC maturation relative to the LPS-induced levels (+, grey diamonds) is indicated by asterisks