Fig. 8
From: R269C variant of ESR1: high prevalence and differential function in a subset of pancreatic cancers
Cell-type dependent effects of R269C-ER on cancer cell proliferation. COLO-357 (a), PANC-1 (b) and MCF-7 (c) cells were transiently transfected with either WT-ER or R269C-ER, seeded in 96-well plates and grown in estrogen-depleted medium and after 24 h were treated with either E2 (10nM) or a vehicle control for 72 h. Viability was assessed using MTT assay and are shown relative to the control WT-ER. Figures show representative results of at least three independent experiments, each performed in hexaplicates, each bar represents the mean ± SD. *, P < 0.05, **, P < 0.01