Fig. 4
From: miR-874 inhibits gastric cancer cell proliferation by targeting SPAG9
miR-874 directly targeted SPAG9 in GC cells. The binding site of miR-874 on the 3′-UTR of SPAG9 (a). Luciferase activity of MCG-823 cells transfected with miR-874 mimics in the presence of pGL3-PIK3CA-SPAG9 Wt or pGL3-PIK3CA-SPAG9 Mut was detected (b). Western blot (c) and RT-qPCR (d) were performed to detect the effect of miR-874 on SPAG9 both at protein level (full-length blots are presented in Additional file 1) and mRNA. ** P < 0.01. GC, gastric cancer