Fig. 6

Apoptotic cell detection. Hematoxylin & eosin (H&E) coloration showing the proliferation zones (yellow drawing) in a. control and b. treated, necrotic zones detected where the film was in contact with the tumor. The black line in A and the yellow arrows represent the position of the implanted film c. Implanted tumor after 8 days of implantation. Pointed arrow represents the drug diffusion direction, yellow drawing represents the necrotic zone and yellow arrow indicates to the implanted film. Immunofluorescence check for the necrotic zone: d- control, there was no detected necrosis; e- Apoptotic parts were detected in the contact zone with the film; and f- Apoptotic zone at higher magnification. Blue represents nucleus, green represents apoptotic cells