Fig. 3

Confirmation of the presence of carboplatin loaded on the film after preparation. a. Control of the detector via the calibration of copper (Cu) as a reference shown in a green peak. b. Film with loaded drug in which two points were checked. Spot B-1 represents the control where no carboplatin was found, but the other elements detected came from the culture medium when the film was incubated: C for carbon, O for oxygen, S for sulfur, Cl for chloride and K for potassium. Spot B-2 represents carboplatin (Pt green peak). The films were covered with a carbon coat before scanning. c, d. Another experiment that performed with a gold (Au) coating layer. We were able to recognize the platinum (Pt) shown in pink peak beside the gold (Au) peak. e. Control film where no drug was loaded onto the film and no detected carboplatin Pt peak. K in the graph is for potassium, N is for nitrogen, C is for carbon, Cl is for chloride, O is for oxygen, Na is for sodium, P is for phosphor. F. Film loaded with drug in the presence of carboplatin after 24 h of incubation and g. after 8 days of incubation. The presence of carboplatin Pt is shown as Pt peak in the corresponding graphs. Y-axis (cps/eV) is counts per second per eV. X-axis (KeV) is kilo electron volts. h. Kinetics of the released carboplatin in the culture medium till 192 h of incubation. Yellow and white zones in a, c, d, e, f and g represent the checked areas with EDX spectroscopy