Fig. 1
From: Total DNA methylation as a biomarker of DNA damage and tumor malignancy in intracranial meningiomas
Flowchart of total genomic m5C estimation. Isolated DNA is hydrolyzed to 3′-mononucleotides (Np, A - adenosine, G - guanosine, C - cytidine, T - thymidine). The hydrolysate is labeled with [γ-32P] ATP, dephosphorylated (detachment of 3′ phosphate), and separated in two dimensions with TLC. The chromatogram is evaluated with phosphoimager, and the spots’ intensities are measured. Those values are used for the calculation of the R coefficient according to the given equation [33]