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Fig. 3 | BMC Cancer

Fig. 3

From: Damage-associated molecular patterns (DAMPs) related to immunogenic cell death are differentially triggered by clinically relevant chemotherapeutics in lung adenocarcinoma cells

Fig. 3

Levels of calreticulin (CRT) exposure and margination in A549 cells treated with chemotherapeutics. a Representative plots of FSC (cell size) versus levels of CRT exposure in the cell surface. Note that axes are in the log scale to allow the observation of shrinked cells (i.e. low FSC cells). Numbers represent the percentage of cells in each quadrant, as described in the legend on the right. On the right is also shown the positive control (CT116 colorectal cancer cells treated with oxaliplatin 25 μM for 48 h). b Representative images of CRT immunocytochemistry. c Margination of CRT. Each dot represents a single cell; red lines represent the average. d Correlation between cell area and CRT margination to the treatments tested. e Total level of CRT in A549 cells treated with chemotherapeutics, obtained from the intensity of red fluorescence measured using the Image Pro Plus 6.0 software. To all graphs from this figure, *p < 0.05; **p < 0.01 and ***p < 0.001 in relation to control (ANOVA followed by Tukey post-hoc)

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