Fig. 5

AML Breg-induced conversion of CD4⁺CD25^- T cells to CD4⁺CD25⁺Foxp3⁺ Tregs. a. Flow cytometry analysis of CD19⁺CD24^highCD38^high Bregs in BM from newly diagnosed AML patients (n = 13). b. The percentage of CD19⁺CD24^highCD38^high Bregs in BM from AML patients (n = 13) was increased compared with those from healthy controls (n = 10). c. The expression of IL-10 and TGF-β in BM Bregs cells had no significant differences between AML patients (n = 3) and controls (n = 4). Data were expressed as Median (P25, P75). d. Normal Bregs could not convert CD4+CD25- T cells into CD4⁺CD25⁺Foxp3⁺ Tregs whether from AML patients (n = 3) or controls (n = 4). AML Bregs could result in higher conversion of CD4⁺CD25^- T cells to CD4⁺CD25⁺Foxp3⁺ Tregs, especially for autologous CD4⁺CD25^- T cells. Data were expressed as mean ± SEM. *P < 0.05, **P < 0.01