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Table 3 Comparison of putative stemness characteristics of parental and hybrid clone cells

From: Comparison of hybrid clones derived from human breast epithelial cells and three different cancer cell lines regarding in vitro cancer stem/ initiating cell properties

 

CD44+/

CD24−/

low

ALDHa

CFAb

MAc

size

MA

count

WBd

SNAIL

WB

SLUG

WB

SOX9

M13SV1-EGFP-Neo

++

+++

+/−

+/−

+/−

++

++

+

HS578T-Hyg

+++

+

+

+/−

+/−

+

+

M13HS-2

+++

+++

++

++

++

++

++

M13HS-8

+++

+

++

+

+

+

++

MDA-MB-435-Hyg

+++

+

+/−

+

M13MDA435–1

+++

+

++

+/−

+/−

+

+

M13MDA435–3

+++

++

++

++

+

++

M13SV1-EGFP-Neo

++

++

+/−

+

+/−

+

+

+/−

MDA-MB-231-Hyg

+++

+/−

+

+

++

M13MDA231–3

+++

+++

+/−

+

+

++

++

M13MDA231–6

+++

++

+/−

+

+/−

++

M13MDA231–11

+++

+++

+/−

+/−

M13MDA231–13

+++

++

+/−

+

++

++

++

  1. a ALDH: ALDH1 activity; b CFA: colony forming assay; c MA: mammosphere assay; d WB: Western blot