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Table 3 Comparison of putative stemness characteristics of parental and hybrid clone cells

From: Comparison of hybrid clones derived from human breast epithelial cells and three different cancer cell lines regarding in vitro cancer stem/ initiating cell properties

  CD44+/
CD24−/
low
ALDHa CFAb MAc
size
MA
count
WBd
SNAIL
WB
SLUG
WB
SOX9
M13SV1-EGFP-Neo ++ +++ +/− +/− +/− ++ ++ +
HS578T-Hyg +++ + + +/− +/− + +
M13HS-2 +++ +++ ++ ++ ++ ++ ++
M13HS-8 +++ + ++ + + + ++
MDA-MB-435-Hyg +++ + +/− +
M13MDA435–1 +++ + ++ +/− +/− + +
M13MDA435–3 +++ ++ ++ ++ + ++
M13SV1-EGFP-Neo ++ ++ +/− + +/− + + +/−
MDA-MB-231-Hyg +++ +/− + + ++
M13MDA231–3 +++ +++ +/− + + ++ ++
M13MDA231–6 +++ ++ +/− + +/− ++
M13MDA231–11 +++ +++ +/− +/−
M13MDA231–13 +++ ++ +/− + ++ ++ ++
  1. a ALDH: ALDH1 activity; b CFA: colony forming assay; c MA: mammosphere assay; d WB: Western blot
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