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Fig. 4 | BMC Cancer

Fig. 4

From: Exploratory analysis of immune checkpoint receptor expression by circulating T cells and tumor specimens in patients receiving neo-adjuvant chemotherapy for operable breast cancer

Fig. 4

Representative images for immuno-histochemical (IHC) analysis of CD8, PD-L1, and PD-1 expression. (a) Representative H&E staining showing a portion of tumor outlined in black and a portion of stroma outlined in red. (b) Representative image showing multicolor IHC staining for all three markers: PD-L1, PD-1, and CD8. Brown staining identifies PD-L1 expression, red identifies PD-1 expression, and green represents CD8 expression. Percentage of cells expressing various markers was determined as the area expressing the marker divided by the total tumor area, total stromal area, or tumor and stromal area together. (c) Additional representative H&E staining showing tumor outlined in black and stroma outlined in red. (d) Representative multicolor IHC staining showing PD-L1 (brown) and CD8 (green) staining in the stroma only with no staining in the tumor. (e) Representative H&E staining. (f) Representative multicolor IHC staining showing only rare CD8 (green) staining within the stroma and no PD-L1 (brown) or PD-1 (red) staining

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